Coding

Part:BBa_K4805012:Design

Designed by: Yuhan Gan   Group: iGEM23_LINKS-China   (2023-10-12)


pPGK1-SaCPR2-tPGK1-pTDH3-CYP736A167-tTDH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1932
    Illegal NheI site found at 2032
    Illegal NheI site found at 5001
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4174
    Illegal BglII site found at 4759
    Illegal XhoI site found at 2818
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2374
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3072
    Illegal BsaI.rc site found at 4184
    Illegal SapI site found at 4303


Design Notes

Its DNA sequences have been optimized which can be expressed in S. cerevisiae.


Source

DNA synthesis

References